We have an Illumina NextSeq 1000 which is capable of generating between 10-360 GB of data which corresponds to 100M to 1.2B reads, depending on your sequencing needs. Read lengths range from 50-600 base pairs.
Estimated Sample Throughput for Key Applications:
Data capabilities for Illumina's NextSeq 1000 platform. Courtesy of Illumina.‡ Using P1 300-cycle kit.
§ Recommended read lengths are 2 × 75 bp for Total RNA Prep and mRNA Prep and 2 × 100 bp for RNA Prep with Enrichment.
** Recommended sequencing depth will largely depend on sample type and experimental objective and will need to be optimized for each study.
†† P1 reagents are a good option for single-cell quality control experiments.
‡‡ Using P1 100-cycle kit.
§§ Using P2 100-cycle kit.
*** A maximum of 384 unique dual indexes is available
| Type of Application | # of samples on a P1 Flow Cell | # of samples on a P2 Flow Cell |
|---|---|---|
| Small Whole-Genome Sequencing (300 cycles) 130 Mb genome; >30x coverage | 7 | 30 |
| Small Whole-Genome Sequencing (600 cycles) 130 Mb genome; > 30x coverage | 15 | 45 |
| Whole Exome Sequencing (200 cycles) 50x mean targeted coverage; 90% targeted coverage at 20x | 4 | 16 |
| Total RNA-Seq (200 cycles) 50M read pairs per sample | 2‡ | 8 |
| mRNA-Seq (200 cycles) 25M read pairs per sample | 4‡ | 16 |
| Single-Cell RNA-Seq (100 cycles) 5K cells, 20K reads/cell | 1†† | 4 |
| miRNA-Seq or Small RNA Analysis (50 cycles) 11M reads/sample | 9‡‡ | 36 |
| 16S RNA Sequencing (600 cycles) | 384*** | 384 |
Data Output Per Flow Cell
| Read Length | Amount of Data Produced with a P1 Flow Cell | Amount of Data Produced with a P2 Flow Cell |
|---|---|---|
| 2 x 50 bp | 10 Gb | 40 Gb |
| 2 x 100 bp | N/A | 80 Gb |
| 2 x 150 bp | 30 Gb | 120 Gb |
| 2 x 300 bp | 60 Gb | 180 Gb |